Updated April 23, 2012!
Lipidomics Symposium - A success! Thank you to our panel of Speakers!
Frontiers in Lipidomics - The Food, Nutrition and Health Connection
March 20, 2012 9 am-4 pm
Cook Campus Center, Rutgers University Cook Campus (Click Here! or go to http://ifnh.rutgers.edu/ to register)
This symposium highlighted the use of mass spectrometry-based lipidomics to answer nutrition and health-related questions.
Agenda:
| 9:00-9:30 |
Registration |
| 9:30-9:45 |
IntroductionPeter Gillies, Ph.D., Director, New Jersey Institute for Food, Nutrition and Health |
| 9:45-10:15 |
The Transition From Lipid Analysis to Lipidomics;Joseph Dixon, Ph.D., Dept. of Nutritional Sciences, Rutgers University |
| 10:15-11:15 |
KEYNOTE ADDRESS: The Macrophage and Plasma Lipidomes in Health, Nutrition, and Disease;Edward Dennis, Ph.D., Dept. of Chemistry and Biochemistry and Dept. of Pharmacology, University of California, San Diego |
| 11:30-12:30 |
Targeted Lipidomic Profiling: A Powerful Nutritional Phenotyping Tool;John Newman, Ph.D., USDA/ARS, Western Human Nutrition Research Center and Dept. of Nutrition, University of California, Davis |
| 12:30-1:30 | Lunch |
| 1:30-2:15 |
Unbiased Analysis of Biological Samples for Lipids and Small Molecule Metabolites;Serhiy Hnatyshyn, Ph.D., Senior Research Scientist, Bristol-Myers Squibb |
| 2:30-3:15 |
What's On the Horizon for Lipidomics;Paul Baker, Ph.D., Lipid Applications Specialist, ABSciex |
| 3:15-3:30 |
Final Summary;George Carman, Ph.D., Dept. of Food Science and Rutgers Center for Lipid Research, Rutgers University |
| 3:30-3:45 |
Funding Opportunities in Lipidomics;Xenia Morin, Ph.D., Office of Grants Facilitation, Rutgers University |
Recent Seminar Presented at the Kern Lipid Meeting in Vail, Colorado on August 7, 2011: "Measuring Lipids in All Comers"- Targeted Lipidomics Mass Spectrometry (Click on Tab - right Margin)!
This seminar covered 3 projects that we are involved in:
1. The disruption in cellular lipids when the enzyme, Phosphatidate Phosphatase (also known as Lipin 1), is missing in Yeast cells. This enzyme has been shown to be an important modulator in obesity!
2 & 3. Measuring lipids in Tobacco leaves (2) and in Diatoms (3) for Bio-fuels!
The Dixon Lab is primarily focused on studying Lipid Metabolism in Liver - especially the regulation of the secretion of Very Low Density Lipoprotein (VLDL). We are also involved in providing lipid measurement expertise to several Biofuels Projects! See Lab News!
Department's LC/MS Lab has been open for 4 years!
The Lab's Mass
Spectrometer: an
Applied Biosystems
4000 Qtrap.
The NIH funded a grant for several investigators performing lipid research at Rutgers University, now organized as the Rutgers Center for Lipid Research (RCLR), to purchase a Liquid Chromatograph Mass Spectrometer (LCMS). The mass spectrometer is dedicated primarily to the research of lipids and lipid metabolism. The goal of the Core lab housing this instrument is to expand the capabilities of these laboratories to study the role of lipids in health and disease and to identify new lipids that are involved in metabolism.
The mass spectrometer is an Applied Biosystems 4000 Q Trap. This instrument is a combination triple quadropole/linear ion trap, a very powerful combination that allows us to perform both highly quantitative and qualitative work. We can use this instrument to act both as a triple quadropole instrument to measure low levels of lipids, and as an ion trap to trap ions in the third quadropole, allowing greater sensitivity and MS3 capabilities to identify new lipids. The possibilities are endless with this instrument.
Ionization Sources
We have several ionization sources to ionize lipids including Electrospray™ and Photospray™. The Electrospray™ source uses high voltage to ionize lipids. The Photospray™ source uses UV radiation, a dopant (usually toluene), and nitrogen to ionize the compounds. We have achieved very high sensitivity for diacylglycerols and sterols with Photospray™, in many cases allowing us to measure 5 pg of lipid per injection.
New Method in April 2011: We can now measure the entire spectrum of fatty acyl CoAs!
We now have the ability to measure fatty acyl CoAs in tissues. This method was established in our lab with the help of Drs. William Blaner and Hongfeng Jiang of Columbia University.
A poster describing our capabilities with the LCMS can be found here.
